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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated G6PD Monoclonal antibody

G6PD Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A7B12

Cat no : CL488-66373

Print datasheet

Synonyms

Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6P, EC:1.1.1.49, 2A7B12

验证数据展示

  • IF Staining of HeLa using CL488-66373

    IF Staining of HeLa using CL488-66373

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 G6PD antibody (CL488-66373, Clone: 2A7B12 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-66373

    FC experiment of HeLa using CL488-66373

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated G6PD Monoclonal antibody (CL488-66373, Clone:2A7B12)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy G6PD Ready-To-Use IHC Kit. G6PD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66373-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66373 targets G6PD in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen G6PD fusion protein Ag21862 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glucose-6-phosphate dehydrogenase
Synonyms Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6P, EC:1.1.1.49, 2A7B12
Calculated Molecular Weight 59 kDa
GenBank Accession NumberBC000337
Gene Symbol G6PD
Gene ID (NCBI) 2539
RRIDAB_2883320
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11413
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 G6PD antibody CL488-66373Download protocol
FC protocol for CL Plus 488 G6PD antibody CL488-66373Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Commun

Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell death

Authors - Michal Scur
View Article