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验证数据展示

  • FC experiment of HeLa using CL647-66373

    FC experiment of HeLa using CL647-66373

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human G6PD (CL647-66373, Clone:2A7B12) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated G6PD Monoclonal antibody

G6PD Monoclonal Antibody for FC (Intra)
Cat No. CL647-66373

产品说明书

CloneNo. 2A7B12

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

G6P, G6PD, G6PD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy G6PD Ready-To-Use IHC Kit. G6PD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66373-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66373 targets G6PD in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 G6PD fusion protein Ag21862 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 glucose-6-phosphate dehydrogenase
别名 G6P, G6PD, G6PD1
计算分子量 59 kDa
观测分子量 60 kDa
GenBank蛋白编号BC000337
基因名称 G6PD
Gene ID (NCBI) 2539
RRIDAB_2920281
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11413
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 G6PD antibody CL647-66373Download protocol
Standard Protocols
Click here to view our Standard Protocols