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验证数据展示

  • IF Staining of HeLa using CL594-66373

    IF Staining of HeLa using CL594-66373

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 G6PD antibody (CL594-66373, Clone: 2A7B12 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-66373

    FC experiment of HeLa using CL594-66373

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human G6PD (CL594-66373, Clone:2A7B12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated G6PD Monoclonal antibody

G6PD Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66373

产品说明书

CloneNo. 2A7B12

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6P, EC:1.1.1.49, 2A7B12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy G6PD Ready-To-Use IHC Kit. G6PD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66373-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66373 targets G6PD in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 G6PD fusion protein Ag21862 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 glucose-6-phosphate dehydrogenase
别名 Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6P, EC:1.1.1.49, 2A7B12
计算分子量 59 kDa
GenBank蛋白编号BC000337
基因名称 G6PD
Gene ID (NCBI) 2539
RRIDAB_2883570
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11413
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL594 G6PD antibody CL594-66373Download protocol
FC protocol for CL594 G6PD antibody CL594-66373Download protocol
Standard Protocols
Click here to view our Standard Protocols