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验证数据展示

  • IF Staining of mouse testis using 20087-1-AP

    IF Staining of mouse testis using 20087-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using GAB4 antibody (20087-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 20087-1-AP

    IF Staining of mouse testis using 20087-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using GAB4 antibody (20087-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 20087-1-AP

    IHC staining of mouse testis using 20087-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20087-1-AP (GAB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 20087-1-AP

    IHC staining of mouse testis using 20087-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20087-1-AP (GAB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GAB4 Polyclonal antibody

GAB4 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 20087-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

GRB2-associated binder 4, GRB2-associated binder 2-like, GRB2 associated binder 4, GRB2 associated binder 2 like, GAB2-like

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20087-1-AP targets GAB4 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GRB2-associated binding protein family, member 4
别名 GRB2-associated binder 4, GRB2-associated binder 2-like, GRB2 associated binder 4, GRB2 associated binder 2 like, GAB2-like
计算分子量 62 kDa
GenBank蛋白编号NM_001037814
基因名称 GAB4
Gene ID (NCBI) 128954
RRIDAB_3669322
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2WGN9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAB4, also named as GAB2-like, belongs to the GAB family. The antibody has no cross reaction to GAB2.

实验方案

Product Specific Protocols
IHC protocol for GAB4 antibody 20087-1-APDownload protocol
IF protocol for GAB4 antibody 20087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols