验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66458 targets GABARAPL1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | GABARAPL1 fusion protein Ag1473 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | GABA(A) receptor-associated protein like 1 |
| Synonyms | APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GEC 1, GEC1 |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 16-18 kDa |
| GenBank Accession Number | BC009309 |
| Gene Symbol | GABARAPL1 |
| Gene ID (NCBI) | 23710 |
| RRID | AB_2919996 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9H0R8 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle and at very low levels in thymus and small intestine.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GABARAPL1 antibody CL594-66458 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |