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GABRA2 Recombinant antibody

GABRA2 Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230158H5

Cat No : 83057-4-RR

Print datasheet

Synonyms

230158H5

验证数据展示

  • FC experiment of HeLa using 83057-4-RR

    FC experiment of HeLa using 83057-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human GABRA2 (83057-4-RR, Clone:230158H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of A549 using 83057-4-RR

    FC experiment of A549 using 83057-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human GABRA2 (83057-4-RR, Clone:230158H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83057-4-RR

    Affinity and Kinetic Characterization of 83057-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83057-4-RR against Human GABRA2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive FC (Intra) detected inHeLa cells, A549 cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83057-4-RR targets GABRA2 in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen GABRA2 fusion protein Ag29301 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 2
Synonyms 230158H5
Calculated Molecular Weight 51 kDa
Observed Molecular Weight50-51 kDa
GenBank Accession NumberNM_000807
Gene Symbol GABRA2
Gene ID (NCBI) 2555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP47869
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.

实验方案

Product Specific Protocols
FC protocol for GABRA2 antibody 83057-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols