• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GAC-specific Polyclonal antibody, PBS Only

GAC-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19958-1-PBS

Print datasheet

Synonyms

GLS, KGA, GLS1, GAM, GAC

验证数据展示

  • WB analysis using 19958-1-AP (same clone as 19958-1-PBS)

    WB analysis using 19958-1-AP (same clone as 19958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

    IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • IP experiment of mouse kidney using 19958-1-AP (same clone as 19958-1-PBS)

    IP experiment of mouse kidney using 19958-1-AP (same clone as 19958-1-PBS)

    IP result of anti-GAC-specific (IP:19958-1-AP, 3ug; Detection:19958-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

    IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • IF Staining of HEK-293 using 19958-1-AP (same clone as 19958-1-PBS)

    IF Staining of HEK-293 using 19958-1-AP (same clone as 19958-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GAC-specific antibody (19958-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KGA/GAC Ready-To-Use IHC Kit. KGA/GAC primary antibody included.

产品信息

19958-1-PBS targets GAC-specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutaminase
Synonyms GLS, KGA, GLS1, GAM, GAC
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberAF158555
Gene Symbol GLS
Gene ID (NCBI) 2744
RRIDAB_10640899
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94925
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.