验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy GAD1 Ready-To-Use IHC Kit. GAD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67648-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67648 targets GAD1 in IF-P applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human, mouse, rat, pig, rabbit |
| 免疫原 | GAD1 fusion protein Ag21336 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glutamate decarboxylase 1 (brain, 67kDa) |
| 别名 | FLJ45882, GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP |
| 计算分子量 | 67 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC002815 |
| 基因名称 | GAD1 |
| Gene ID (NCBI) | 2571 |
| RRID | AB_3084368 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99259 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development(PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GAD1 antibody CL488-67648 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |