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验证数据展示

  • WB analysis using 83703-2-RR (same clone as 83703-2-PBS)

    WB analysis using 83703-2-RR (same clone as 83703-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83703-2-RR (GALNT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83703-2-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83703-2-RR (same clone as 83703-2-PBS)

    IHC staining of human ovary cancer using 83703-2-RR (same clone as 83703-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83703-2-RR (GALNT2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83703-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GALNT2 antibody included
  • Cytometric bead array standard curve of MP00666-1

    Cytometric bead array standard curve of MP00666-1

    Cytometric bead array standard curve of MP00666-1, GALNT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83703-1-PBS. Detection antibody: 83703-2-PBS. Standard: Ag11523. Range: 1.563-100 ng/mL.

  • Affinity and Kinetic Characterization of 83703-2-RR

    Affinity and Kinetic Characterization of 83703-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83703-2-RR against Human GALNT2 were performed. The affinity constant is 2.04 nM.

GALNT2 Recombinant antibody, PBS Only (Detector)

GALNT2 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 83703-2-PBS

产品说明书

CloneNo. 240723G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2, EC:2.4.1.41

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GALNT2 Ready-To-Use IHC Kit. GALNT2 primary antibody included.

产品信息

83703-2-PBS targets GALNT2 as part of a matched antibody pair:

MP00666-1: 83703-1-PBS capture and 83703-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 GALNT2 fusion protein Ag11523 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
别名 Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2, EC:2.4.1.41
计算分子量 571 aa, 65 kDa
观测分子量65 kDa
GenBank蛋白编号BC041120
基因名称 GALNT2
Gene ID (NCBI) 2590
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ10471
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.