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验证数据展示

  • WB analysis using 83703-2-RR

    WB analysis using 83703-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83703-2-RR (GALNT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83703-2-RR

    IHC staining of human ovary cancer using 83703-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83703-2-RR (GALNT2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GALNT2 antibody included
  • Affinity and Kinetic Characterization of 83703-2-RR

    Affinity and Kinetic Characterization of 83703-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83703-2-RR against Human GALNT2 were performed. The affinity constant is 2.04 nM.

GALNT2 Recombinant antibody

GALNT2 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83703-2-RR

产品说明书

CloneNo. 240723G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2, EC:2.4.1.41

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MKN-45 cells, A549 cells, U-87 MG cells, HuH-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GALNT2 Ready-To-Use IHC Kit. GALNT2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83703-2-RR targets GALNT2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 GALNT2 fusion protein Ag11523 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
别名 Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2, EC:2.4.1.41
计算分子量 571 aa, 65 kDa
观测分子量65 kDa
GenBank蛋白编号BC041120
基因名称 GALNT2
Gene ID (NCBI) 2590
RRIDAB_3671305
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ10471
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.

实验方案

Product Specific Protocols
WB protocol for GALNT2 antibody 83703-2-RRDownload protocol
IHC protocol for GALNT2 antibody 83703-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols