CoraLite® Plus 647-conjugated GAPDH (Human Specific) Recombinant antibody
GAPDH (Human Specific) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
2G21
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-80570 targets GAPDH (Human Specific) in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | GAPDH (Human Specific) fusion protein Ag0766 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | glyceraldehyde-3-phosphate dehydrogenase |
Synonyms | GAPDH, GAPD, EC:2.6.99.-, EC:1.2.1.12, CDABP0047 |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 33 kDa |
GenBank Accession Number | BC004109 |
Gene Symbol | GAPDH |
Gene ID (NCBI) | 2597 |
RRID | AB_2920334 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P04406 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 647 GAPDH (Human Specific) antibody CL647-80570 | Download protocol |
IF protocol for CL Plus 647 GAPDH (Human Specific) antibody CL647-80570 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |