• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82971-1-RR (same clone as 82971-1-PBS)

    WB analysis using 82971-1-RR (same clone as 82971-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82971-1-RR (GARNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 82971-1-RR (same clone as 82971-1-PBS)

    FC experiment of U2OS using 82971-1-RR (same clone as 82971-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug GARNL1 Recombinant antibody (82971-1-RR, Clone:230165F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82971-1-RR (same clone as 82971-1-PBS)

    WB analysis of HEK-293 using 82971-1-RR (same clone as 82971-1-PBS)

    WB result of GARNL1 antibody (82971-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARNL1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82971-1-RR

    Affinity and Kinetic Characterization of 82971-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82971-1-RR against Human GARNL1 were performed. The affinity constant is 99.6 pM.

  • Featured Product
  • KD/KO验证

GARNL1 Recombinant antibody, PBS Only

GARNL1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82971-1-PBS

产品说明书

CloneNo. 230165F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy GARNL1 Ready-To-Use IHC Kit. GARNL1 primary antibody included.

产品信息

82971-1-PBS targets GARNL1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 GARNL1 fusion protein Ag31518 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GTPase activating Rap/RanGAP domain-like 1
别名 Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE
计算分子量230KD
观测分子量260 kDa
GenBank蛋白编号NM_014990.3
基因名称 GARNL1
Gene ID (NCBI) 253959
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6GYQ0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).