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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82971-1-RR (same clone as 82971-1-PBS)

    WB analysis using 82971-1-RR (same clone as 82971-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82971-1-RR (GARNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 82971-1-RR (same clone as 82971-1-PBS)

    FC experiment of U2OS using 82971-1-RR (same clone as 82971-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug GARNL1 Recombinant antibody (82971-1-RR, Clone:230165F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82971-1-RR (same clone as 82971-1-PBS)

    WB analysis of HEK-293 using 82971-1-RR (same clone as 82971-1-PBS)

    WB result of GARNL1 antibody (82971-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARNL1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82971-1-RR

    Affinity and Kinetic Characterization of 82971-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82971-1-RR against Human GARNL1 were performed. The affinity constant is 99.6 pM.

  • Featured Product
  • KD/KO Validated

GARNL1 Recombinant antibody, PBS Only

GARNL1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82971-1-PBS

Print datasheet

CloneNo. 230165F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GARNL1 Ready-To-Use IHC Kit. GARNL1 primary antibody included.

产品信息

82971-1-PBS targets GARNL1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen GARNL1 fusion protein Ag31518 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GTPase activating Rap/RanGAP domain-like 1
Synonyms Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE
Calculated Molecular Weight230KD
Observed Molecular Weight260 kDa
GenBank Accession NumberNM_014990.3
Gene Symbol GARNL1
Gene ID (NCBI) 253959
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6GYQ0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).