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验证数据展示

  • WB analysis using 82971-1-RR

    WB analysis using 82971-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82971-1-RR (GARNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 82971-1-RR

    FC experiment of U2OS using 82971-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug GARNL1 Recombinant antibody (82971-1-RR, Clone:230165F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HEK-293 using 82971-1-RR

    WB analysis of HEK-293 using 82971-1-RR

    WB result of GARNL1 antibody (82971-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARNL1 transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 82971-1-RR

    Affinity and Kinetic Characterization of 82971-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82971-1-RR against Human GARNL1 were performed. The affinity constant is 99.6 pM.

  • Featured Product
  • KD/KO Validated

GARNL1 Recombinant antibody

GARNL1 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82971-1-RR

Print datasheet

CloneNo. 230165F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy GARNL1 Ready-To-Use IHC Kit. GARNL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82971-1-RR targets GARNL1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen GARNL1 fusion protein Ag31518 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GTPase activating Rap/RanGAP domain-like 1
Synonyms Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE
Calculated Molecular Weight230KD
Observed Molecular Weight260 kDa
GenBank Accession NumberNM_014990.3
Gene Symbol GARNL1
Gene ID (NCBI) 253959
RRIDAB_3670719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6GYQ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).

实验方案

Product Specific Protocols
WB protocol for GARNL1 antibody 82971-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols