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验证数据展示

  • WB analysis using 82971-1-RR

    WB analysis using 82971-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82971-1-RR (GARNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 82971-1-RR

    FC experiment of U2OS using 82971-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug GARNL1 Recombinant antibody (82971-1-RR, Clone:230165F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HEK-293 using 82971-1-RR

    WB analysis of HEK-293 using 82971-1-RR

    WB result of GARNL1 antibody (82971-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARNL1 transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 82971-1-RR

    Affinity and Kinetic Characterization of 82971-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82971-1-RR against Human GARNL1 were performed. The affinity constant is 99.6 pM.

  • Featured Product
  • KD/KO验证

GARNL1 Recombinant antibody

GARNL1 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82971-1-RR

产品说明书

CloneNo. 230165F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy GARNL1 Ready-To-Use IHC Kit. GARNL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82971-1-RR targets GARNL1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 GARNL1 fusion protein Ag31518 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GTPase activating Rap/RanGAP domain-like 1
别名 Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE
计算分子量230KD
观测分子量260 kDa
GenBank蛋白编号NM_014990.3
基因名称 GARNL1
Gene ID (NCBI) 253959
RRIDAB_3670719
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6GYQ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).

实验方案

Product Specific Protocols
WB protocol for GARNL1 antibody 82971-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols