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验证数据展示

  • FC experiment of U2OS using CL488-82971

    FC experiment of U2OS using CL488-82971

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Garnl1 Recombinant Antibody (CL488-82971, Clone:230165F11)(red), or 0.4 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated GARNL1 Recombinant antibody

GARNL1 Recombinant Antibody for FC (Intra)
Cat No. CL488-82971

Print datasheet

CloneNo. 230165F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy GARNL1 Ready-To-Use IHC Kit. GARNL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82971-1-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82971 targets GARNL1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen GARNL1 fusion protein Ag31518 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GTPase activating Rap/RanGAP domain-like 1
Synonyms Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE
Calculated Molecular Weight230KD
Observed Molecular Weight260 kDa
GenBank Accession NumberNM_014990.3
Gene Symbol GARNL1
Gene ID (NCBI) 253959
RRIDAB_3673158
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6GYQ0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GARNL1 antibody CL488-82971Download protocol
Standard Protocols
Click here to view our Standard Protocols