验证数据展示
经过测试的应用
Positive FC (Intra) detected in | U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-82971 targets GARNL1 in FC (Intra) applications and shows reactivity with human samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | GARNL1 fusion protein Ag31518 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | GTPase activating Rap/RanGAP domain-like 1 |
别名 | Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE |
计算分子量 | 230KD |
观测分子量 | 260 kDa |
GenBank蛋白编号 | NM_014990.3 |
基因名称 | GARNL1 |
Gene ID (NCBI) | 253959 |
RRID | AB_3673158 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q6GYQ0 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 GARNL1 antibody CL488-82971 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |