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验证数据展示

  • WB analysis using 67893-1-Ig (same clone as 67893-1-PBS)

    WB analysis using 67893-1-Ig (same clone as 67893-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67893-1-Ig (GARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67893-1-Ig (same clone as 67893-1-PBS)

    WB analysis of HepG2 using 67893-1-Ig (same clone as 67893-1-PBS)

    WB result of GARS antibody (67893-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARS transfected HepG2 cells. This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 67893-1-Ig (same clone as 67893-1-PBS)

    IHC staining of human skin cancer using 67893-1-Ig (same clone as 67893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67893-1-Ig (GARS antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GARS antibody included
  • IF Staining of A431 using 67893-1-Ig (same clone as 67893-1-PBS)

    IF Staining of A431 using 67893-1-Ig (same clone as 67893-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GARS antibody (67893-1-Ig, Clone: 2H9D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GARS Monoclonal antibody, PBS Only

GARS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67893-1-PBS

产品说明书

CloneNo. 2H9D3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GARS Ready-To-Use IHC Kit. GARS primary antibody included.

产品信息

67893-1-PBS targets GARS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 GARS fusion protein Ag8432 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 glycyl-tRNA synthetase
别名 AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1
计算分子量 739 aa, 83 kDa
观测分子量75-80 kDa
GenBank蛋白编号BC007755
基因名称 GARS
Gene ID (NCBI) 2617
RRIDAB_2918649
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP41250
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycyl-tRNA synthetase 1 (GARS, GeneID:2617) is one of the aminoacyl-tRNA synthetases. It is essential for protein synthesis in all cells and tissues (PMID: 17035524) and charges tRNA molecules with glycine by catalyzing the ATP-dependent ligation of glycine to the 3'-end of it's cognate tRNA via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PMID: 17544401, PMID: 28675565, PMID: 24898252) . GARS is located in cytoplasm, but also can be secreted from cells and as an extracellular protein which may influence cell signaling. Mutation of GARS causes peripheral neuropathies such as CMT2D, SMA (PMID: 26503042, PMID: 32181591) , or associates with cancer. Two transcript variants encoding different isoforms have been found for this gene. The molecular weight of isoform2 is about 78 kDa.