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IHCeasy GARS Ready-To-Use IHC Kit

GARS Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC0879

Synonyms

AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0879 (GARS IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0879 (GARS IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0879 (GARS IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0879 (GARS IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using KHC0879 (GARS IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using KHC0879 (GARS IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using KHC0879 (GARS IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using KHC0879 (GARS IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0879 (GARS IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0879 (GARS IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0879 (GARS IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0879 (GARS IHC Kit).

  • Components in IHCeasy ready-to-use IHC kit.

Product Information

KHC0879 is a ready-to-use IHC kit for staining of GARS. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy GARS Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Mouse Monoclonal
Secondary antibody type Polymer-HRP-Goat anti-Mouse
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

Glycyl-tRNA synthetase 1 (GARS) is one of the aminoacyl-tRNA synthetases. It is essential for protein synthesis in all cells and tissues and charges tRNA molecules with glycine by catalyzing the ATP-dependent ligation of glycine to the 3'-end of it's cognate tRNA via the formation of an aminoacyl-adenylate intermediate(Gly-AMP). GARS is located in cytoplasm, but also can be secreted from cells and as an extracellular protein which may influence cell signaling. Mutation of GARS causes peripheral neuropathies such as CMT2D, SMA, or associates with cancer. Two transcript variants encoding different isoforms have been found for this gene.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1