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GAS2L1 Monoclonal antibody

GAS2L1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C6D5

Cat no : 68051-1-Ig

Print datasheet

Synonyms

GAR22, GAS2 like protein 1, GAS2L1, growth arrest 2 like 1

验证数据展示

  • WB analysis using 68051-1-Ig

    WB analysis using 68051-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of mouse testis using 68051-1-Ig

    IHC staining of mouse testis using 68051-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, mouse testis tissue, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68051-1-Ig targets GAS2L1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen GAS2L1 fusion protein Ag31232 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name growth arrest-specific 2 like 1
Synonyms GAR22, GAS2 like protein 1, GAS2L1, growth arrest 2 like 1
Calculated Molecular Weight 73 kDa
Observed Molecular Weight73 kDa
GenBank Accession NumberBC011047
Gene Symbol GAS2L1
Gene ID (NCBI) 10634
RRIDAB_2918793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99501
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAS2L1(growth arrest‐specific 2‐like 1) is a component of the cytoskeleton that mediates the interaction between microtubules and microfilament (PMID: 34555082). GAS2L1 contains an N‐terminal actin‐binding calponin homology (CH) domain, a MT lattice‐binding Gas2‐related (GAR) domain and a C‐terminal SxIP motif, which mediates the interaction with MT plus ends via EB proteins. GAS2L1 regulates microtubule dynamics and stability by interacting with microtubule plus-end tracking proteins, such as MAPRE1, to regulate microtubule growth along actin stress fibers (PubMed:24706950).

实验方案

Product Specific Protocols
WB protocol for GAS2L1 antibody 68051-1-IgDownload protocol
IHC protocol for GAS2L1 antibody 68051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols