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验证数据展示

  • FC experiment of Jurkat using CL488-60011

    FC experiment of Jurkat using CL488-60011

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GATA1 (CL488-60011, Clone:5E2A8) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated GATA1 Monoclonal antibody

GATA1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-60011

产品说明书

CloneNo. 5E2A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

FC (Intra)

别名

GATA1, GATA-1, GATA-binding factor 1, GF1, GF-1, NFE1, NF-E1 DNA-binding protein, XLTT, 5E2A8, ERYF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 60011-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60011 targets GATA1 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 GATA1 fusion protein Ag1350 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GATA binding protein 1 (globin transcription factor 1)
别名 GATA1, GATA-1, GATA-binding factor 1, GF1, GF-1, NFE1, NF-E1 DNA-binding protein, XLTT, 5E2A8, ERYF1
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC009797
基因名称 GATA1
Gene ID (NCBI) 2623
RRIDAB_2919226
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP15976
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GATA1 antibody CL488-60011Download protocol
Standard Protocols
Click here to view our Standard Protocols