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GATA2 Monoclonal antibody

GATA2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2B5A1

Cat no : 67722-1-Ig

Print datasheet

Synonyms

NFE1B, GATA-binding protein 2, GATA binding protein 2, Endothelial transcription factor GATA-2, 2B5A1

验证数据展示

  • WB analysis using 67722-1-Ig

    WB analysis using 67722-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of K-562 using 67722-1-Ig

    FC experiment of K-562 using 67722-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human GATA2 (67722-1-Ig, Clone:2B5A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of SH-SY5Y using 67722-1-Ig

    IF Staining of SH-SY5Y using 67722-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GATA2 antibody (67722-1-Ig, Clone: 2B5A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HUVEC cells, LNCaP cells, PC-12 cells, HepG2 cells, HEK-293 cells, K-562 cells, NIH/3T3 cells, bEnd.3 cells
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy GATA2 Ready-To-Use IHC Kit. GATA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67722-1-Ig targets GATA2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GATA2 fusion protein Ag1557 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name GATA binding protein 2
Synonyms NFE1B, GATA-binding protein 2, GATA binding protein 2, Endothelial transcription factor GATA-2, 2B5A1
Observed Molecular Weight50 kDa
GenBank Accession NumberBC018988
Gene Symbol GATA2
Gene ID (NCBI) 2624
ENSEMBL Gene IDENSG00000179348
RRIDAB_2882909
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23769
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.

实验方案

Product Specific Protocols
WB protocol for GATA2 antibody 67722-1-IgDownload protocol
IF protocol for GATA2 antibody 67722-1-IgDownload protocol
FC protocol for GATA2 antibody 67722-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols