• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of SH-SY5Y using CL488-67722

    IF Staining of SH-SY5Y using CL488-67722

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 GATA2 antibody (CL488-67722, Clone: 2B5A1 ) at dilution of 1:200.

  • FC experiment of K-562 using CL488-67722

    FC experiment of K-562 using CL488-67722

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GATA2 (CL488-67722, Clone:2B5A1) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated GATA2 Monoclonal antibody

GATA2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67722

产品说明书

CloneNo. 2B5A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

NFE1B, GATA-binding protein 2, GATA binding protein 2, Endothelial transcription factor GATA-2, 2B5A1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy GATA2 Ready-To-Use IHC Kit. GATA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67722-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67722 targets GATA2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 GATA2 fusion protein Ag1557 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GATA binding protein 2
别名 NFE1B, GATA-binding protein 2, GATA binding protein 2, Endothelial transcription factor GATA-2, 2B5A1
观测分子量50 kDa
GenBank蛋白编号BC018988
基因名称 GATA2
Gene ID (NCBI) 2624
ENSEMBL Gene IDENSG00000179348
RRIDAB_2919527
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP23769
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GATA2 antibody CL488-67722Download protocol
FC protocol for CL Plus 488 GATA2 antibody CL488-67722Download protocol
Standard Protocols
Click here to view our Standard Protocols