验证数据展示
经过测试的应用
| Positive WB detected in | pig kidney tissue, rat kidney tissue, mouse kidney tissue, pig pancreas tissue, rat pancreas tissue, mouse pancreas tissue |
Planning an IHC experiment? We recommend our IHCeasy GATM Ready-To-Use IHC Kit. GATM primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68513-1-Ig targets GATM in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | GATM fusion protein Ag3754 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glycine amidinotransferase (L-arginine:glycine amidinotransferase) |
| 别名 | Transamidinase, L-arginine:glycine amidinotransferase, EC:2.1.4.1, AGAT, 1D12G5 |
| 计算分子量 | 423 aa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC004141 |
| 基因名称 | GATM |
| Gene ID (NCBI) | 2628 |
| RRID | AB_3085222 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P50440 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GATM antibody 68513-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |