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GATM Monoclonal antibody

GATM Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D12G5

Cat no : 68513-1-Ig

Print datasheet

Synonyms

Transamidinase, L-arginine:glycine amidinotransferase, EC:2.1.4.1, AGAT, 1D12G5

验证数据展示

  • WB analysis of pig kidney using 68513-1-Ig

    WB analysis of pig kidney using 68513-1-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68513-1-Ig (GATM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68513-1-Ig

    WB analysis using 68513-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68513-1-Ig (GATM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inpig kidney tissue, rat kidney tissue, mouse kidney tissue, pig pancreas tissue, rat pancreas tissue, mouse pancreas tissue
Planning an IHC experiment? We recommend our IHCeasy GATM Ready-To-Use IHC Kit. GATM primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68513-1-Ig targets GATM in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen GATM fusion protein Ag3754 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycine amidinotransferase (L-arginine:glycine amidinotransferase)
Synonyms Transamidinase, L-arginine:glycine amidinotransferase, EC:2.1.4.1, AGAT, 1D12G5
Calculated Molecular Weight 423 aa, 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC004141
Gene Symbol GATM
Gene ID (NCBI) 2628
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP50440
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.

实验方案

Product Specific Protocols
WB protocol for GATM antibody 68513-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols