验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human placenta tissue, HUVEC cells, pig spleen tissue, rat spleen tissue, HEK-293 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67161-1-Ig targets GBP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, rat, pig |
| 文献引用反应性 | human, mouse, chinese tree shrews |
| 免疫原 | GBP1 fusion protein Ag7767 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | GTP binding protein 1 |
| 别名 | Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1, EC:3.6.1.- |
| 计算分子量 | 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC002666 |
| 基因名称 | GBP1 |
| Gene ID (NCBI) | 2633 |
| RRID | AB_2882457 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P32455 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II IFNs and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GBP1 antibody 67161-1-Ig | Download protocol |
| IHC protocol for GBP1 antibody 67161-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol GBP1 Facilitates Indoleamine 2,3-Dioxygenase Extracellular Secretion to Promote the Malignant Progression of Lung Cancer. | ||
J Immunol Tupaia GBP1 Interacts with STING to Initiate Autophagy and Restrict Herpes Simplex Virus Type 1 Infection. | ||
Vet Microbiol Insights into the effect of guanylate-binding protein 1 on the survival of Brucella intracellularly
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