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GBX2 Polyclonal antibody

GBX2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 21639-1-AP

Print datasheet

Synonyms

Homeobox protein GBX-2, Gastrulation and brain-specific homeobox protein 2

验证数据展示

  • WB analysis of HeLa using 21639-1-AP

    WB analysis of HeLa using 21639-1-AP

    WB result of GBX2 antibody (21639-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected HeLa cells.

  • IF Staining of HeLa using 21639-1-AP

    IF Staining of HeLa using 21639-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBX2 antibody (21639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • WB analysis of mouse stomach using 21639-1-AP

    WB analysis of mouse stomach using 21639-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21639-1-AP (GBX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 21639-1-AP

    IP experiment of HeLa using 21639-1-AP

    IP result of anti-GBX2 (IP:21639-1-AP, 4ug; Detection:21639-1-AP 1:300) with HeLa cells lysate 3000ug.

经过测试的应用

Positive WB detected inmouse stomach tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21639-1-AP targets GBX2 in WB, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, chIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen GBX2 fusion protein Ag16288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gastrulation brain homeobox 2
Synonyms Homeobox protein GBX-2, Gastrulation and brain-specific homeobox protein 2
Calculated Molecular Weight 348 aa, 37 kDa
Observed Molecular Weight 33-37 kDa
GenBank Accession NumberBC137448
Gene Symbol GBX2
Gene ID (NCBI) 2637
RRIDAB_2878896
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52951
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homeobox protein GBX-2 (GBX-2) is a homeobox gene essential for normal development of the midbrain and the anterior hindbrain. It mainly expressed in the brain, and located in nucleoplasm. It is presumably a transcription factor that is likely to regulate the expression of genes involved in establishing early A/P pattern in the neural plate (PMID: 9247335).

实验方案

Product Specific Protocols
WB protocol for GBX2 antibody 21639-1-APDownload protocol
IF protocol for GBX2 antibody 21639-1-APDownload protocol
IP protocol for GBX2 antibody 21639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders

Authors - David Shin
View Article
humanchIP

RNA Biol

NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.

Authors - Xiwen Li
View Article
humanWB,ChIP

Funct Integr Genomics

Oncogenic GBX2 promotes the malignant behaviors of bladder cancer cells by binding to the ITGA5 promoter and activating its transcription.

Authors - Yaoyao Xiong
View Article