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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GBX2 Polyclonal antibody

GBX2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21639

Print datasheet

Synonyms

验证数据展示

  • IF Staining of HeLa using CL488-21639

    IF Staining of HeLa using CL488-21639

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GBX2 antibody (CL488-21639) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 21639-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21639 targets GBX2 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen GBX2 fusion protein Ag16288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gastrulation brain homeobox 2
Synonyms
Calculated Molecular Weight 348 aa, 37 kDa
Observed Molecular Weight 33-37 kDa
GenBank Accession NumberBC137448
Gene Symbol GBX2
Gene ID (NCBI) 2637
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52951
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homeobox protein GBX-2 (GBX-2) is a homeobox gene essential for normal development of the midbrain and the anterior hindbrain. It mainly expressed in the brain, and located in nucleoplasm. It is presumably a transcription factor that is likely to regulate the expression of genes involved in establishing early A/P pattern in the neural plate (PMID: 9247335).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GBX2 antibody CL488-21639Download protocol
Standard Protocols
Click here to view our Standard Protocols