• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GCAT Polyclonal antibody

GCAT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16317-1-AP

Print datasheet

Synonyms

AKB ligase, Aminoacetone synthase, GCAT, Glycine acetyltransferase, KBL

验证数据展示

  • WB analysis using 16317-1-AP

    WB analysis using 16317-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16317-1-AP (GCAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 16317-1-AP

    IHC staining of human pancreas using 16317-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 16317-1-AP (GCAT antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas using 16317-1-AP

    IHC staining of human pancreas using 16317-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 16317-1-AP (GCAT antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16317-1-AP targets GCAT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen GCAT fusion protein Ag9367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase)
Synonyms AKB ligase, Aminoacetone synthase, GCAT, Glycine acetyltransferase, KBL
Calculated Molecular Weight 419 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC014457
Gene Symbol GCAT
Gene ID (NCBI) 23464
RRIDAB_2263046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75600
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GCAT antibody 16317-1-APDownload protocol
IHC protocol for GCAT antibody 16317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Life Sci Alliance

Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.

Authors - Shengya Tian
View Article