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GCGR Recombinant antibody

GCGR Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241501C4

Cat no : 84237-5-RR

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 84237-5-RR

    WB analysis using 84237-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84237-5-RR (GCGR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84237-5-RR targets GCGR in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GCGR fusion protein Ag24861 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glucagon receptor
Synonyms
Calculated Molecular Weight 477 aa, 54 kDa
Observed Molecular Weight62-68 kDa
GenBank Accession NumberBC104854
Gene Symbol GCGR
Gene ID (NCBI) 2642
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP47871
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucagon receptor (GCGR) is a secretin-like (class B) family of G-protein coupled receptors (GPCRs). It plays an important role in elevating the glucose concentration in the blood and has thus become one of the promising therapeutic targets for treating type 2 diabetes mellitus (PMID: 26236379). GCGR is a 62-kDa glycoprotein that contains at least four N-linked oligosaccharide chains (PMID: 2174441; 15245877). Defects in the gene of GCGR cause non-insulin-dependent diabetes mellitus (NIDDM).

实验方案

Product Specific Protocols
WB protocol for GCGR antibody 84237-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols