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  • Featured Product
  • KD/KO Validated

GCHFR Polyclonal antibody

GCHFR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18809-1-AP

Print datasheet

Synonyms

GCHFR, GFRP, HsT16933, P35

验证数据展示

  • WB analysis using 18809-1-AP

    WB analysis using 18809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18809-1-AP (GCHFR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervix using 18809-1-AP

    IHC staining of human cervix using 18809-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 18809-1-AP (GCHFR antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human cervix using 18809-1-AP

    IHC staining of human cervix using 18809-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 18809-1-AP (GCHFR antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18809-1-AP targets GCHFR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTP cyclohydrolase I feedback regulator
Synonyms GCHFR, GFRP, HsT16933, P35
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC112262
Gene Symbol GCHFR
Gene ID (NCBI) 2644
RRIDAB_10596912
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30047
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCHFR,also named as p35 and GFRP, belongs to the GFRP family. GCHFR mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. It modulates BH4 synthesis and oxidative stress. This inhibition is reversed by L-phenylalanine.

实验方案

Product Specific Protocols
WB protocol for GCHFR antibody 18809-1-APDownload protocol
IHC protocol for GCHFR antibody 18809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Antioxid Redox Signal

Characterization of Transgenic Gfrp Knock-In Mice: Implications for Tetrahydrobiopterin in Modulation of Normal Tissue Radiation Responses.

Authors - Pathak Rupak R
  • KO Validated
View Article