验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human cervix tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
18809-1-AP targets GCHFR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GTP cyclohydrolase I feedback regulator |
| 别名 | GCHFR, GFRP, HsT16933, P35 |
| 计算分子量 | 10 kDa |
| 观测分子量 | 10 kDa |
| GenBank蛋白编号 | BC112262 |
| 基因名称 | GCHFR |
| Gene ID (NCBI) | 2644 |
| RRID | AB_10596912 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30047 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GCHFR,also named as p35 and GFRP, belongs to the GFRP family. GCHFR mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. It modulates BH4 synthesis and oxidative stress. This inhibition is reversed by L-phenylalanine.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GCHFR antibody 18809-1-AP | Download protocol |
| IHC protocol for GCHFR antibody 18809-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |