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  • Featured Product
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GCLM Recombinant antibody, PBS Only

GCLM Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4K16

Cat no : 82623-1-PBS

Print datasheet

Synonyms

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

验证数据展示

  • WB analysis using 82623-1-RR (same clone as 82623-1-PBS)

    WB analysis using 82623-1-RR (same clone as 82623-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82623-1-RR (same clone as 82623-1-PBS)

    WB analysis of HeLa using 82623-1-RR (same clone as 82623-1-PBS)

    WB result of GCLM antibody (82623-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82623-1-RR (same clone as 82623-1-PBS)

    IHC staining of mouse brain using 82623-1-RR (same clone as 82623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82623-1-RR

    Affinity and Kinetic Characterization of 82623-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82623-1-RR against Human GCLM were performed. The affinity constant is below 1 pM.

产品信息

82623-1-PBS targets GCLM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GCLM fusion protein Ag5497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutamate-cysteine ligase, modifier subunit
Synonyms Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit
Calculated Molecular Weight 31 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC041809
Gene Symbol GCLM
Gene ID (NCBI) 2730
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48507
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.