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验证数据展示

  • WB analysis using 82623-1-RR

    WB analysis using 82623-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82623-1-RR

    WB analysis of HeLa using 82623-1-RR

    WB result of GCLM antibody (82623-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells.

  • IHC staining of mouse brain using 82623-1-RR

    IHC staining of mouse brain using 82623-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82623-1-RR

    Affinity and Kinetic Characterization of 82623-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82623-1-RR against Human GCLM were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

GCLM Recombinant antibody

GCLM Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82623-1-RR

产品说明书

CloneNo. 4K16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, RAW 264.7 cells, HEK-293 cells, A431 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human ovarian cancer, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82623-1-RR targets GCLM in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GCLM fusion protein Ag5497 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutamate-cysteine ligase, modifier subunit
别名 Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit
计算分子量 31 kDa
观测分子量31 kDa
GenBank蛋白编号BC041809
基因名称 GCLM
Gene ID (NCBI) 2730
RRIDAB_3086493
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48507
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.

实验方案

Product Specific Protocols
WB protocol for GCLM antibody 82623-1-RRDownload protocol
IHC protocol for GCLM antibody 82623-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols