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验证数据展示

  • WB analysis using 68768-1-Ig

    WB analysis using 68768-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68768-1-Ig

    IF Staining of HeLa using 68768-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GDF15 antibody (68768-1-Ig, Clone: 1B1H4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

GDF-15 Monoclonal antibody

GDF-15 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68768-1-Ig

Print datasheet

CloneNo. 1B1H4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GDF15, MIC-1, Growth/differentiation factor 15, growth differentiation factor 15, GDF 15

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, Caki-1 cells, HepG2 cells, LNCaP cells, HT-29 cells, human placenta tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68768-1-Ig targets GDF-15 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen GDF-15 fusion protein Ag33750 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name growth differentiation factor 15
Synonyms GDF15, MIC-1, Growth/differentiation factor 15, growth differentiation factor 15, GDF 15
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC008962
Gene Symbol GDF15
Gene ID (NCBI) 9518
RRIDAB_3670423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99988
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

实验方案

Product Specific Protocols
WB protocol for GDF-15 antibody 68768-1-IgDownload protocol
IF protocol for GDF-15 antibody 68768-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols