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CoraLite® Plus 488-conjugated GDF5 Recombinant antibody

GDF5 Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240944E5

Cat no : CL488-83988-4

Print datasheet

Synonyms

验证数据展示

  • IF Staining of RAW 264.7 using CL488-83988-4

    IF Staining of RAW 264.7 using CL488-83988-4

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CoraLite® Plus 488 GDF5 antibody (CL488-83988-4, Clone: 240944E5 ) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inRAW 264.7 cells
For other applications, we recommend the unconjugated version of this antibody, 83988-4-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-83988-4 is GDF5 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen GDF5 fusion protein Ag6238 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name growth differentiation factor 5
Synonyms
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC032495
Gene Symbol GDF5
Gene ID (NCBI) 8200
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43026
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth and Differentiation Factor 5 (GDF5) plays important roles during joint formation. It is one of the earliest genes expressed in the embryonic joint interzone7-10, fated to give rise to joint tissues including articular cartilage, synovium, menisci, and ligaments.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GDF5 antibody CL488-83988-4Download protocol
Standard Protocols
Click here to view our Standard Protocols