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Host
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Species Reactivity
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Species Reactivity
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Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

    WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

    WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • WB analysis of U-251 using 66434-1-Ig (same clone as 66434-1-PBS)

    WB analysis of U-251 using 66434-1-Ig (same clone as 66434-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

    IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

    IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66434-1-Ig (same clone as 66434-1-PBS)

    IF Staining of MCF-7 using 66434-1-Ig (same clone as 66434-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66434-1-Ig (GDI1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

GDI1 Monoclonal antibody, PBS Only

GDI1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66434-1-PBS

产品说明书

CloneNo. 4E1G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

OPHN2, RABGDIA, XAP4, MRX41, GDP dissociation inhibitor 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66434-1-PBS targets GDI1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 GDI1 fusion protein Ag17511 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GDP dissociation inhibitor 1
别名 OPHN2, RABGDIA, XAP4, MRX41, GDP dissociation inhibitor 1
计算分子量 51 kDa
观测分子量 55 kDa
GenBank蛋白编号BC000317
基因名称 GDI1
Gene ID (NCBI) 2664
RRIDAB_2881804
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP31150
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55 kDa protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034)