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GDI1 Monoclonal antibody, PBS Only

GDI1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E1G1

Cat no : 66434-1-PBS

Print datasheet

Synonyms

OPHN2, RABGDIA, XAP4, MRX41, GDP dissociation inhibitor 1

验证数据展示

  • WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

    WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

    WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • WB analysis of U-251 using 66434-1-Ig (same clone as 66434-1-PBS)

    WB analysis of U-251 using 66434-1-Ig (same clone as 66434-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

    IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

    IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66434-1-Ig (same clone as 66434-1-PBS)

    IF Staining of MCF-7 using 66434-1-Ig (same clone as 66434-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66434-1-Ig (GDI1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

产品信息

66434-1-PBS targets GDI1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen GDI1 fusion protein Ag17511 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name GDP dissociation inhibitor 1
Synonyms OPHN2, RABGDIA, XAP4, MRX41, GDP dissociation inhibitor 1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC000317
Gene Symbol GDI1
Gene ID (NCBI) 2664
RRIDAB_2881804
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP31150
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55 kDa protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034)