验证数据展示
经过测试的应用
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | SH-SY5Y cells |
Positive FC (Intra) detected in | SH-SY5Y cells, U-251 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:125-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83087-1-RR targets GDNF in IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | GDNF fusion protein Ag24068 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | glial cell derived neurotrophic factor |
别名 | Glial cell line-derived neurotrophic factor, ATF2, ATF1, ATF, Astrocyte-derived trophic factor |
计算分子量 | 211 aa, 24 kDa |
观测分子量 | 32-40 kDa |
GenBank蛋白编号 | BC069369 |
基因名称 | GDNF |
Gene ID (NCBI) | 2668 |
RRID | AB_3670802 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P39905 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glial-derived neurotrophic factor (GDNF) is a member of the transforming growth factor (TGF)-α family and exhibits potent neuroprotective activity. GDNF is a potent promoter of neuronal survival in the CNS and peripheral nervous system (PNS). It has a relatively high speciicity for dopaminergic neurons and thus become an efective treatment for Parkinson's diseases (PD), with clinical trials currently in progress. Protein dimers of GDNF have a reported molecular weight of 32-40 kDa(PMID: 17982417, PMID: 23251488, PMID: 11429269).
实验方案
Product Specific Protocols | |
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IHC protocol for GDNF antibody 83087-1-RR | Download protocol |
IF protocol for GDNF antibody 83087-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |