验证数据展示
经过测试的应用
| Positive IF/ICC detected in | SH-SY5Y cells |
For other applications, we recommend the unconjugated version of this antibody, 83087-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83087 targets GDNF in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | GDNF fusion protein Ag24068 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | glial cell derived neurotrophic factor |
| Synonyms | |
| Calculated Molecular Weight | 211 aa, 24 kDa |
| Observed Molecular Weight | 32-40 kDa |
| GenBank Accession Number | BC069369 |
| Gene Symbol | GDNF |
| Gene ID (NCBI) | 2668 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P39905 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glial-derived neurotrophic factor (GDNF) is a member of the transforming growth factor (TGF)-α family and exhibits potent neuroprotective activity. GDNF is a potent promoter of neuronal survival in the CNS and peripheral nervous system (PNS). It has a relatively high speciicity for dopaminergic neurons and thus become an efective treatment for Parkinson's diseases (PD), with clinical trials currently in progress. Protein dimers of GDNF have a reported molecular weight of 32-40 kDa(PMID: 17982417, PMID: 23251488, PMID: 11429269).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GDNF antibody CL488-83087 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |