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GDPD5 Polyclonal antibody

GDPD5 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16494-1-AP

Print datasheet

Synonyms

GDE2, GDPD5, PP1665, PP9363, UNQ1850/PRO3580

验证数据展示

  • IHC staining of human spleen using 16494-1-AP

    IHC staining of human spleen using 16494-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 16494-1-AP

    IHC staining of human spleen using 16494-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of mouse skeletal muscle using 16494-1-AP

    IP experiment of mouse skeletal muscle using 16494-1-AP

    IP result of anti-GDPD5 (IP:16494-1-AP, 4ug; Detection:16494-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

经过测试的应用

Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman spleen tissue, human kidney tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16494-1-AP targets GDPD5 in IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GDPD5 fusion protein Ag9622 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycerophosphodiester phosphodiesterase domain containing 5
Synonyms GDE2, GDPD5, PP1665, PP9363, UNQ1850/PRO3580
Calculated Molecular Weight 127 aa, 13 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC020203
Gene Symbol GDPD5
Gene ID (NCBI) 81544
RRIDAB_2111683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WTR4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDPD5(Glycerophosphodiester phosphodiesterase domain-containing protein 5) is also named as GDE2 and belongs to the glycerophosphoryl diester phosphodiesterase family. The deduced sequence of GDE2 contains 607 amino acids with seven putative transmembrane regions. GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC(PMID:18667693). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IHC protocol for GDPD5 antibody 16494-1-APDownload protocol
IP protocol for GDPD5 antibody 16494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols