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验证数据展示

  • IHC staining of human spleen using 16494-1-AP

    IHC staining of human spleen using 16494-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 16494-1-AP

    IHC staining of human spleen using 16494-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of mouse skeletal muscle using 16494-1-AP

    IP experiment of mouse skeletal muscle using 16494-1-AP

    IP result of anti-GDPD5 (IP:16494-1-AP, 4ug; Detection:16494-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

GDPD5 Polyclonal antibody

GDPD5 Polyclonal Antibody for IP, IHC, ELISA
Cat No. 16494-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IP, IHC, ELISA

别名

GDE2, GDPD5, PP1665, PP9363, UNQ1850/PRO3580

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman spleen tissue, human kidney tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16494-1-AP targets GDPD5 in IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GDPD5 fusion protein Ag9622 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycerophosphodiester phosphodiesterase domain containing 5
别名 GDE2, GDPD5, PP1665, PP9363, UNQ1850/PRO3580
计算分子量 127 aa, 13 kDa
观测分子量 52 kDa
GenBank蛋白编号BC020203
基因名称 GDPD5
Gene ID (NCBI) 81544
RRIDAB_2111683
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WTR4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDPD5(Glycerophosphodiester phosphodiesterase domain-containing protein 5) is also named as GDE2 and belongs to the glycerophosphoryl diester phosphodiesterase family. The deduced sequence of GDE2 contains 607 amino acids with seven putative transmembrane regions. GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC(PMID:18667693). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IHC protocol for GDPD5 antibody 16494-1-APDownload protocol
IP protocol for GDPD5 antibody 16494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols