验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, human brain tissue, mouse brain tissue, rat brain tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human heart tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66012-1-Ig targets GEFT in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human |
| 免疫原 | GEFT fusion protein Ag10819 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RhoA/RAC/CDC42 exchange factor |
| 别名 | ARHGEF25, RhoA/RAC/CDC42 exchange factor, Rho guanine nucleotide exchange factor 25, p63RhoGEF |
| 计算分子量 | 64 kDa |
| 观测分子量 | 53 kDa, 63 kDa |
| GenBank蛋白编号 | BC012860 |
| 基因名称 | GEFT |
| Gene ID (NCBI) | 115557 |
| RRID | AB_11044192 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q86VW2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GEFT antibody 66012-1-Ig | Download protocol |
| IHC protocol for GEFT antibody 66012-1-Ig | Download protocol |
| IF protocol for GEFT antibody 66012-1-Ig | Download protocol |
| FC protocol for GEFT antibody 66012-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
