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Host
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Species Reactivity
0
Species Reactivity
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis of mouse skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

    WB analysis of mouse skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66012-1-Ig (same clone as 66012-1-PBS)

    FC experiment of HEK-293 using 66012-1-Ig (same clone as 66012-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug GEFT Monoclonal antibody (66012-1-Ig, Clone:5A11F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66012-1-Ig (same clone as 66012-1-PBS)

    IF Staining of HepG2 using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GEFT antibody (66012-1-Ig, Clone: 5A11F8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

GEFT Monoclonal antibody, PBS Only

GEFT Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66012-1-PBS

产品说明书

CloneNo. 5A11F8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

ARHGEF25, RhoA/RAC/CDC42 exchange factor, Rho guanine nucleotide exchange factor 25, p63RhoGEF

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66012-1-PBS targets GEFT in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 GEFT fusion protein Ag10819 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RhoA/RAC/CDC42 exchange factor
别名 ARHGEF25, RhoA/RAC/CDC42 exchange factor, Rho guanine nucleotide exchange factor 25, p63RhoGEF
计算分子量64 kDa
观测分子量 53 kDa, 63 kDa
GenBank蛋白编号BC012860
基因名称 GEFT
Gene ID (NCBI) 115557
RRIDAB_11044192
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86VW2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68kDa).