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GEFT Monoclonal antibody, PBS Only

GEFT Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A11F8

Cat No : 66012-1-PBS

Print datasheet

Synonyms

ARHGEF25, GEFT, p63RhoGEF, RhoA/RAC/CDC42 exchange factor

验证数据展示

  • WB analysis of mouse skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

    WB analysis of mouse skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66012-1-Ig (same clone as 66012-1-PBS)

    FC experiment of HEK-293 using 66012-1-Ig (same clone as 66012-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug GEFT Monoclonal antibody (66012-1-Ig, Clone:5A11F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

产品信息

66012-1-PBS targets GEFT in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, FC, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen GEFT fusion protein Ag10819 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RhoA/RAC/CDC42 exchange factor
Synonyms ARHGEF25, GEFT, p63RhoGEF, RhoA/RAC/CDC42 exchange factor
Calculated Molecular Weight64 kDa
Observed Molecular Weight 53 kDa, 63 kDa
GenBank Accession NumberBC012860
Gene Symbol GEFT
Gene ID (NCBI) 115557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86VW2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68kDa).