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GEMIN5 Recombinant antibody

GEMIN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241205G7

Cat no : 84171-5-RR

Print datasheet

Synonyms

Gem-associated protein 5, Gem associated protein 5, 241205G7

验证数据展示

  • WB analysis using 84171-5-RR

    WB analysis using 84171-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84171-5-RR (GEMIN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84171-5-RR

    IF Staining of HeLa using 84171-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GEMIN5 antibody (84171-5-RR, Clone: 241205G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HEK-293 using 84171-5-RR

    FC experiment of HEK-293 using 84171-5-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GEMIN5 Recombinant antibody (84171-5-RR, Clone:241205G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84171-5-RR

    Affinity and Kinetic Characterization of 84171-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84171-5-RR against Human GEMIN5 were performed. The affinity constant is 0.619 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, COLO 320 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84171-5-RR is GEMIN5 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen GEMIN5 fusion protein Ag19220 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gem (nuclear organelle) associated protein 5
Synonyms Gem-associated protein 5, Gem associated protein 5, 241205G7
Calculated Molecular Weight 1508 aa, 169 kDa
Observed Molecular Weight 168-180 kDa
GenBank Accession NumberBC113614
Gene Symbol GEMIN5
Gene ID (NCBI) 25929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8TEQ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.

实验方案

Product Specific Protocols
WB protocol for GEMIN5 antibody 84171-5-RRDownload protocol
IF protocol for GEMIN5 antibody 84171-5-RRDownload protocol
FC protocol for GEMIN5 antibody 84171-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols