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验证数据展示

  • WB analysis using 84171-5-RR

    WB analysis using 84171-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84171-5-RR (GEMIN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84171-5-RR

    IF Staining of HeLa using 84171-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GEMIN5 antibody (84171-5-RR, Clone: 241205G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HEK-293 using 84171-5-RR

    FC experiment of HEK-293 using 84171-5-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GEMIN5 Recombinant antibody (84171-5-RR, Clone:241205G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84171-5-RR

    Affinity and Kinetic Characterization of 84171-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84171-5-RR against Human GEMIN5 were performed. The affinity constant is 0.619 nM.

GEMIN5 Recombinant antibody

GEMIN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84171-5-RR

产品说明书

CloneNo. 241205G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Gem-associated protein 5, Gem associated protein 5, 241205G7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, COLO 320 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy GEMIN5 Ready-To-Use IHC Kit. GEMIN5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84171-5-RR targets GEMIN5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 GEMIN5 fusion protein Ag19220 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 gem (nuclear organelle) associated protein 5
别名 Gem-associated protein 5, Gem associated protein 5, 241205G7
计算分子量 1508 aa, 169 kDa
观测分子量 168-180 kDa
GenBank蛋白编号BC113614
基因名称 GEMIN5
Gene ID (NCBI) 25929
RRIDAB_3671728
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TEQ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.

实验方案

Product Specific Protocols
WB protocol for GEMIN5 antibody 84171-5-RRDownload protocol
IF protocol for GEMIN5 antibody 84171-5-RRDownload protocol
FC protocol for GEMIN5 antibody 84171-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols