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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84171-5-RR (same clone as 84171-5-PBS)

    WB analysis using 84171-5-RR (same clone as 84171-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84171-5-RR (GEMIN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84171-5-RR (same clone as 84171-5-PBS)

    IF Staining of HeLa using 84171-5-RR (same clone as 84171-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GEMIN5 antibody (84171-5-RR, Clone: 241205G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 84171-5-RR (same clone as 84171-5-PBS)

    FC experiment of HEK-293 using 84171-5-RR (same clone as 84171-5-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GEMIN5 Recombinant antibody (84171-5-RR, Clone:241205G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84171-5-RR

    Affinity and Kinetic Characterization of 84171-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84171-5-RR against Human GEMIN5 were performed. The affinity constant is 0.619 nM.

GEMIN5 Recombinant antibody, PBS Only

GEMIN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84171-5-PBS

产品说明书

CloneNo. 241205G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

Gem-associated protein 5, Gem associated protein 5, 241205G7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GEMIN5 Ready-To-Use IHC Kit. GEMIN5 primary antibody included.

产品信息

84171-5-PBS targets GEMIN5 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 GEMIN5 fusion protein Ag19220 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 gem (nuclear organelle) associated protein 5
别名 Gem-associated protein 5, Gem associated protein 5, 241205G7
计算分子量 1508 aa, 169 kDa
观测分子量 168-180 kDa
GenBank蛋白编号BC113614
基因名称 GEMIN5
Gene ID (NCBI) 25929
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TEQ6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.