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验证数据展示

  • IF Staining of HeLa using CL488-24897

    IF Staining of HeLa using CL488-24897

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GEMIN5 antibody (CL488-24897) at dilution of 1:200.

CoraLite® Plus 488-conjugated GEMIN5 Polyclonal antibody

GEMIN5 Polyclonal Antibody for IF/ICC
Cat No. CL488-24897

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

DKFZp586M1824, Gem associated protein 5, GEMIN5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GEMIN5 Ready-To-Use IHC Kit. GEMIN5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24897-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24897 targets GEMIN5 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 GEMIN5 fusion protein Ag19268 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gem (nuclear organelle) associated protein 5
别名 DKFZp586M1824, Gem associated protein 5, GEMIN5
计算分子量 1508 aa, 169 kDa
观测分子量 168 kDa
GenBank蛋白编号BC113614
基因名称 GEMIN5
Gene ID (NCBI) 25929
RRIDAB_3084085
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEQ6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GEMIN5 antibody CL488-24897Download protocol
Standard Protocols
Click here to view our Standard Protocols