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GET4 Polyclonal antibody

GET4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27768-1-AP

Print datasheet

Synonyms

C7orf20, CEE, CGI 20, GET4, Transmembrane domain recognition complex 35 kDa subunit, TRC35

验证数据展示

  • IHC staining of human colon using 27768-1-AP

    IHC staining of human colon using 27768-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27768-1-AP (GET4 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with GET4 antibody included
  • WB analysis using 27768-1-AP

    WB analysis using 27768-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27768-1-AP (GET4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 27768-1-AP

    WB analysis of HEK-293 using 27768-1-AP

    HEK-293 cells and MCF-7 cells were subjected to SDS PAGE followed by western blot with 27768-1-AP (GET4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, HeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GET4 Ready-To-Use IHC Kit. GET4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

27768-1-AP targets GET4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen GET4 fusion protein Ag26904 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 7 open reading frame 20
Synonyms C7orf20, CEE, CGI 20, GET4, Transmembrane domain recognition complex 35 kDa subunit, TRC35
Calculated Molecular Weight 327 aa, 37 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC003550
Gene Symbol GET4
Gene ID (NCBI) 51608
RRIDAB_2880967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L5D6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GET4 antibody 27768-1-APDownload protocol
IHC protocol for GET4 antibody 27768-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

SMN-primed ribosomes modulate the translation of transcripts related to spinal muscular atrophy.

Authors - Fabio Lauria
View Article
humanWB

EMBO Rep

C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains

Authors - Julia Jülg
View Article