验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy GFAP Ready-To-Use IHC Kit. GFAP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81063-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81063 targets GFAP in IF-P applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | GFAP fusion protein Ag10423 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | glial fibrillary acidic protein |
| Synonyms | |
| Calculated Molecular Weight | 432 aa, 50 kDa |
| GenBank Accession Number | BC013596 |
| Gene Symbol | GFAP |
| Gene ID (NCBI) | 2670 |
| RRID | AB_3673042 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P14136 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GFAP antibody CL488-81063 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |