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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis of Recombinant protein using 81835-2-RR (same clone as 81835-2-PBS)

    WB analysis of Recombinant protein using 81835-2-RR (same clone as 81835-2-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 81835-2-RR (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81835-2-PBS in a different storage buffer formulation.

  • WB analysis of Transfected HEK-293 using 81835-2-RR (same clone as 81835-2-PBS)

    WB analysis of Transfected HEK-293 using 81835-2-RR (same clone as 81835-2-PBS)

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81835-2-RR (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81835-2-PBS in a different storage buffer formulation.

  • IF Staining of Transfected COS-7 using 81835-2-RR (same clone as 81835-2-PBS)

    IF Staining of Transfected COS-7 using 81835-2-RR (same clone as 81835-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected COS-7 cells using GFP tag antibody (81835-2-RR, Clone: 241614B3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81835-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81835-2-RR

    Affinity and Kinetic Characterization of 81835-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81835-2-RR against Aequorea victoria GFP tag were performed. The affinity constant is 0.619 nM.

Aequorea victoria GFP tag Recombinant antibody, PBS Only

GFP tag Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 81835-2-PBS
CloneNo. 241614B3

Host / Isotype

Rabbit / IgG

Reactivity

aequorea victoria, recombinant protein

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

241614B3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

81835-2-PBS targets GFP tag in WB, IF/ICC, Indirect ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity aequorea victoria, recombinant protein
Immunogen GFP tag fusion protein Ag2128 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GFP tag
Synonyms 241614B3
Calculated Molecular Weight 26 kDa
GenBank Accession NumberM62653
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP42212
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm. When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.