验证数据展示
经过测试的应用
| Positive WB detected in | mouse cerebellum tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28335-1-AP targets GFRAL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | GFRAL fusion protein Ag28504 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GDNF family receptor alpha like |
| 别名 | UNQ9356, GRAL, C6orf144, bA360D14.1 |
| 计算分子量 | 45 aa, 85 kDa |
| 观测分子量 | 45-60 kDa |
| GenBank蛋白编号 | NM_207410 |
| 基因名称 | GFRAL |
| Gene ID (NCBI) | 389400 |
| RRID | AB_3669653 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GFRAL (GDNF family receptor alpha-like), also called C6orf144, is the brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses (PMID: 28846097, 28846098, 28846099, 28953886, 36630958). The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues (PMID: 32661391). GFRAL is a single-pass membrane protein, and localizes to the plasma membrane. It is mainly expressed in the hindbrain, and its mainstream molecular weight is 45-60 kd.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GFRAL antibody 28335-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |