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GHRHR Polyclonal antibody

GHRHR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20715-1-AP

Print datasheet

Synonyms

GHRFR, GHRH receptor, GHRHR, GHRHRpsv, GRF receptor, GRFR

验证数据展示

  • WB analysis of HEK-293 using 20715-1-AP

    WB analysis of HEK-293 using 20715-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20715-1-AP (GHRHR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20715-1-AP

    IHC staining of human kidney using 20715-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20715-1-AP (GHRHR Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 20715-1-AP

    IHC staining of human kidney using 20715-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20715-1-AP (GHRHR Antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

20715-1-AP targets GHRHR in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GHRH receptor
Synonyms GHRFR, GHRH receptor, GHRHR, GHRHRpsv, GRF receptor, GRFR
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberNM_000823
Gene Symbol GHRHR
Gene ID (NCBI) 2692
RRIDAB_11232423
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02643
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GHRHR, also named GRFR, belongs to the G-protein coupled receptor 2 family. It is a GRF receptor coupled to G proteins that activate adenylyl cyclase. GHRHR stimulates somatotroph cell growth, GH gene transcription, and GH secretion. The antibody is specific to GHRHR. The antibody is specific to GHRHR.

实验方案

Product Specific Protocols
WB protocol for GHRHR antibody 20715-1-APDownload protocol
IHC protocol for GHRHR antibody 20715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Neural Regen Res

Growth hormone promotes the reconstruction of injured axons in the hypothalamo-neurohypophyseal system

Authors - Kai Li
View Article