验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy GIGYF2 Ready-To-Use IHC Kit. GIGYF2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24790-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-24790 targets GIGYF2 in IF/ICC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | GIGYF2 fusion protein Ag20329 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GRB10 interacting GYF protein 2 |
| 别名 | TNRC15, PERQ2, PERQ amino acid-rich with GYF domain-containing protein 2, KIAA0642, GYF2 |
| 计算分子量 | 1320 aa, 152 kDa |
| 观测分子量 | 150-170 kDa |
| GenBank蛋白编号 | BC146775 |
| 基因名称 | GIGYF2 |
| Gene ID (NCBI) | 26058 |
| RRID | AB_3672773 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6Y7W6 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GIGYF2 (GRB10 interacting GYF protein 2) was initially identified through its interaction with GRB10, an adapter protein that binds activated INS-like growth factor (IGF-I) and INS receptors (PMID: 12771153; 19744960). It may act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. The gene of GIGYF2 has been proposed as the Parkinson-disease (PD) gene underlying the PARK11 locus (PMID: 18358451).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GIGYF2 antibody CL488-24790 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |