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验证数据展示

  • IHC staining of human heart using 13445-1-AP

    IHC staining of human heart using 13445-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13445-1-AP (GIMAP2 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human heart using 13445-1-AP

    IHC staining of human heart using 13445-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13445-1-AP (GIMAP2 antibody) at dilution of 1:200 (under 10x lens).

  • WB analysis of human heart using 13445-1-AP

    WB analysis of human heart using 13445-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13445-1-AP (GIMAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

GIMAP2 Polyclonal antibody

GIMAP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13445-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

GIMAP2, GTPase IMAP family member 2, GTPase, IMAP family member 2, HIMAP2, IMAP2, Immunity associated protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, human kidney tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13445-1-AP targets GIMAP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 GIMAP2 fusion protein Ag4273 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTPase, IMAP family member 2
别名 GIMAP2, GTPase IMAP family member 2, GTPase, IMAP family member 2, HIMAP2, IMAP2, Immunity associated protein 2
计算分子量 337 aa, 38 kDa
观测分子量 28-40 kDa
GenBank蛋白编号BC032345
基因名称 GIMAP2
Gene ID (NCBI) 26157
RRIDAB_2111871
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UG22
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This protein was not eliminated clearly. It belongs to GTPase of the immunity-associated protein family. One of its homologous protein, GIMAP1, runs as a 30kd protein in SDS-PAGE, lower than the calculated molecular weight. This antibody detected one 28kd band in several tissues.

实验方案

Product Specific Protocols
WB protocol for GIMAP2 antibody 13445-1-APDownload protocol
IHC protocol for GIMAP2 antibody 13445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols