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GIMAP5 Monoclonal antibody

GIMAP5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B6F2

Cat no : 66943-1-Ig

Print datasheet

Synonyms

FLJ11296, GIMAP5, GTPase IMAP family member 5, GTPase, IMAP family member 5, hIAN5, HIMAP3, IAN 5, IAN4, IAN4L1, IAN5, IMAP3, Immunity associated protein 3

验证数据展示

  • WB analysis of Jurkat using 66943-1-Ig

    WB analysis of Jurkat using 66943-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66943-1-Ig (GIMAP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 66943-1-Ig

    IHC staining of human lymphoma using 66943-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66943-1-Ig (GIMAP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 66943-1-Ig

    IHC staining of human lymphoma using 66943-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66943-1-Ig (GIMAP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66943-1-Ig targets GIMAP5 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen GIMAP5 fusion protein Ag18045 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name GTPase, IMAP family member 5
Synonyms FLJ11296, GIMAP5, GTPase IMAP family member 5, GTPase, IMAP family member 5, hIAN5, HIMAP3, IAN 5, IAN4, IAN4L1, IAN5, IMAP3, Immunity associated protein 3
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC011732
Gene Symbol GIMAP5
Gene ID (NCBI) 55340
RRIDAB_2882267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96F15
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GIMAP5 gene encodes immunity-associated nucleotide 5 protein (IAN5) belonging to GTPase immune-associated protein subfamily. GIMAP5 is widely expressed with high levels in lymph node and spleen. The loss of Gimap5 (GTPase of the 5) gene function is the underlying cause of lymphopenia and autoimmune diabetes in the BioBreeding (BB) rat. Mouse gimap5 is necessary for the survival of peripheral T cells, NK/NKT-cell development, hematopoietic progenitor cell survival and the maintenance of normal liver function. GIMAP5 may regulate T lymphocyte survival by mechanisms that operate upstream of mitochondria.

实验方案

Product Specific Protocols
WB protocol for GIMAP5 antibody 66943-1-IgDownload protocol
IHC protocol for GIMAP5 antibody 66943-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols