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GIPC1 Polyclonal antibody
GIPC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, mouse skeletal muscle tissue, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, A2780 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:600-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14822-1-AP targets GIPC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | GIPC1 fusion protein Ag6597 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | GIPC PDZ domain containing family, member 1 |
Synonyms | RGS19IP1, RGS19-interacting protein 1, PDZ domain-containing protein GIPC1, GIPC, GAIP C-terminus-interacting protein |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC000410 |
Gene Symbol | GIPC1 |
Gene ID (NCBI) | 10755 |
RRID | AB_2263269 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O14908 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for GIPC1 antibody 14822-1-AP | Download protocol |
IHC protocol for GIPC1 antibody 14822-1-AP | Download protocol |
IF protocol for GIPC1 antibody 14822-1-AP | Download protocol |
IP protocol for GIPC1 antibody 14822-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Am J Hum Genet Expansion of GGC Repeat in GIPC1 Is Associated with Oculopharyngodistal Myopathy. | ||
Dev Cell SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt. | ||
Pain G protein-coupled receptor GPR151 is involved in trigeminal neuropathic pain through the induction of Gβγ/extracellular signal-regulated kinase-mediated neuroinflammation in the trigeminal ganglion. | ||
FASEB J BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.
| ||
CNS Neurosci Ther G-alpha interacting protein interacting protein, C terminus 1 regulates epileptogenesis by increasing the expression of metabotropic glutamate receptor 7. |