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  • KD/KO Validated

GIPC1 Polyclonal antibody

GIPC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14822-1-AP

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Synonyms

RGS19IP1, RGS19-interacting protein 1, PDZ domain-containing protein GIPC1, GIPC, GAIP C-terminus-interacting protein



经过测试的应用

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A2780 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:600-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14822-1-AP targets GIPC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen GIPC1 fusion protein Ag6597 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GIPC PDZ domain containing family, member 1
Synonyms RGS19IP1, RGS19-interacting protein 1, PDZ domain-containing protein GIPC1, GIPC, GAIP C-terminus-interacting protein
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC000410
Gene Symbol GIPC1
Gene ID (NCBI) 10755
RRIDAB_2263269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAIP interacting protein C terminus, member 1(GIPC1), also known as PDZ domain-containing protein GIPC1, is a cytoplasmic protein that also localizes to the peripheral membrane, acting as an adaptor protein linking receptor interactions to intracellular signaling pathways, including cell cycle regulation, and expression has been associated with some cancers (PMID: 19175293, 26757732). This antibody is specific to GIPC1 with a band at 42 kDa (PMID: 18721486).

实验方案

Product Specific Protocols
WB protocol for GIPC1 antibody 14822-1-APDownload protocol
IHC protocol for GIPC1 antibody 14822-1-APDownload protocol
IF protocol for GIPC1 antibody 14822-1-APDownload protocol
IP protocol for GIPC1 antibody 14822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Am J Hum Genet

Expansion of GGC Repeat in GIPC1 Is Associated with Oculopharyngodistal Myopathy.

Authors - Jianwen Deng
humanIHC

Dev Cell

SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt.

Authors - Christoph J Burckhardt
humanWB

Ann Neurol

GIPC1 CGG repeat expansion is associated with movement disorders.

Authors - Yu Fan
mouseWB

Pain

G protein-coupled receptor GPR151 is involved in trigeminal neuropathic pain through the induction of Gβγ/extracellular signal-regulated kinase-mediated neuroinflammation in the trigeminal ganglion.

Authors - Bao-Chun Jiang
mouseWB

FASEB J

BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.

Authors - Nahoko Eiraku
  • KD Validated
human,mouseWB

CNS Neurosci Ther

G-alpha interacting protein interacting protein, C terminus 1 regulates epileptogenesis by increasing the expression of metabotropic glutamate receptor 7.

Authors - Yong Liu