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验证数据展示

  • IF Staining of HepG2 using CL488-14822

    IF Staining of HepG2 using CL488-14822

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GIPC1 antibody (CL488-14822) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GIPC1 Polyclonal antibody

GIPC1 Polyclonal Antibody for IF/ICC
Cat No. CL488-14822

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

C19orf3, GIPC, GIPC1, GLUT1CBP, Hs.6454, IIP 1, NIP, RGS GAIP interacting protein, RGS19 interacting protein 1, RGS19IP1, SEMCAP, SYNECTIIN, SYNECTIN, Tax interaction protein 2, TIP 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 14822-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14822 targets GIPC1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen GIPC1 fusion protein Ag6597 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GIPC PDZ domain containing family, member 1
Synonyms C19orf3, GIPC, GIPC1, GLUT1CBP, Hs.6454, IIP 1, NIP, RGS GAIP interacting protein, RGS19 interacting protein 1, RGS19IP1, SEMCAP, SYNECTIIN, SYNECTIN, Tax interaction protein 2, TIP 2
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC000410
Gene Symbol GIPC1
Gene ID (NCBI) 10755
RRIDAB_3672608
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14908
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAIP interacting protein C terminus, member 1(GIPC1), also known as PDZ domain-containing protein GIPC1, is a cytoplasmic protein that also localizes to the peripheral membrane, acting as an adaptor protein linking receptor interactions to intracellular signaling pathways, including cell cycle regulation, and expression has been associated with some cancers (PMID: 19175293, 26757732). This antibody is specific to GIPC1 with a band at 42 kDa (PMID: 18721486).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GIPC1 antibody CL488-14822Download protocol
FC protocol for CL Plus 488 GIPC1 antibody CL488-14822Download protocol
Standard Protocols
Click here to view our Standard Protocols